{"id":781,"date":"2019-09-06T12:48:31","date_gmt":"2019-09-06T12:48:31","guid":{"rendered":"http:\/\/blogs.rollins.edu\/ship\/?p=781"},"modified":"2019-09-06T12:48:31","modified_gmt":"2019-09-06T12:48:31","slug":"alexandria-juffre-conducts-research-internship-on-cancer-at-tufts-university-in-boston-ma-may-july-2019-blog-2","status":"publish","type":"post","link":"https:\/\/blogs.rollins.edu\/ship\/2019\/09\/06\/alexandria-juffre-conducts-research-internship-on-cancer-at-tufts-university-in-boston-ma-may-july-2019-blog-2\/","title":{"rendered":"Alexandria Juffre Conducts Research Internship on Cancer at Tufts University in Boston, MA (May-July, 2019) Blog 2"},"content":{"rendered":"

Blog 2: Research <\/strong><\/p>\n

My arrival at the lab was at the very start of a new mice model experiment the doctor and his post-doctoral researchers were beginning. Each of these experiments at this lab last a total of 26 weeks, so my time at the lab of 10 weeks was not sufficient to help them with that experiment in particular. Instead, in the lab, I was doing additional research to help back up their findings in their previous mice study which was researching the impact of a particular gut bacteria on the presence and progression of colorectal cancer. The first three weeks I was there, the post-doc researcher, Gar Yee, helped teach me the lab protocol, but she moved to work at the University of Florida on the fourth week and I learned independently from then on.<\/p>\n

\"\"My lab space I worked in while in Boston. The grey incubator on the left housed my cells and the fridge on the right housed reagents and solutions needed for experiments.<\/p>\n

During my time, I gained experience independently in cell culture assays, growing and splitting colorectal cancer cells and hTLR-4 cells, along with PCR analysis, ELISA assays, Caspase-3 assays, TLR-4 activation assays, as well as assisting with Western Blot analysis, immunohistochemistry, and TGF-beta activation assays. Additionally, my analysis of TLR-4 activation led to a partnership in which I analyzed samples from a lab at Northeastern University to help identify the effects of certain bacteria on cell lines. My time at the USDA HRNCA was also supplemented with visits to 2 conferences and multiple speakers to heard about advancements in nutritional and cancer research, as well as to see my boss present on his findings at our lab. The experiments I learned and knowledge I gained during this experience was an excellent addition to my curriculum at Rollins and will aid my future lab work in Biology and Biochemistry practices.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Blog 2: Research My arrival at the lab was at the very start of a new mice model experiment the doctor and his post-doctoral researchers were beginning. Each of these experiments at this lab last a total of 26 weeks, … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-781","post","type-post","status-publish","format-standard","hentry","category-internship"],"_links":{"self":[{"href":"https:\/\/blogs.rollins.edu\/ship\/wp-json\/wp\/v2\/posts\/781","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.rollins.edu\/ship\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.rollins.edu\/ship\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.rollins.edu\/ship\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.rollins.edu\/ship\/wp-json\/wp\/v2\/comments?post=781"}],"version-history":[{"count":1,"href":"https:\/\/blogs.rollins.edu\/ship\/wp-json\/wp\/v2\/posts\/781\/revisions"}],"predecessor-version":[{"id":782,"href":"https:\/\/blogs.rollins.edu\/ship\/wp-json\/wp\/v2\/posts\/781\/revisions\/782"}],"wp:attachment":[{"href":"https:\/\/blogs.rollins.edu\/ship\/wp-json\/wp\/v2\/media?parent=781"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.rollins.edu\/ship\/wp-json\/wp\/v2\/categories?post=781"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.rollins.edu\/ship\/wp-json\/wp\/v2\/tags?post=781"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}